產(chǎn)品貨號(hào)XY0015-1
產(chǎn)品名稱
ErbB-2抗體蛋白名
Receptor tyrosine-protein kinase erbB-2
別稱NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
ErbB-2抗體免疫原多肽
來源兔
特異性抗人
克隆類型多克隆
應(yīng)用IH(P)免疫組化(石蠟);IH(F)免疫組化(冰凍);IC細(xì)胞免疫化學(xué)
成分200μg/ml親和純化抗體,0.01M PBS,30%甘油。
保存條件連續(xù)使用時(shí)4°C存儲(chǔ),保質(zhì)期六個(gè)月;長期存儲(chǔ)時(shí)建議分裝為10ul以上小包裝-20°C存儲(chǔ),并避免反復(fù)凍融,保質(zhì)期一年。
背景資料This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
參考效價(jià):Western blotting: 1:100-400
Immunocytochemistry in fixed cells: 1:100-500
Immunohistochemistry in frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
ErbB-2抗體的保存建議:
抗體保存得當(dāng)與否直接決定了抗體的活性和使用效果。如果抗體保存得當(dāng),大部分抗體活性都可以維持?jǐn)?shù)月甚至數(shù)年。請(qǐng)按照說明書或者抗體標(biāo)簽上推薦的保存條件正確保存抗體!無論是保存還是運(yùn)輸,絕對(duì)避免反復(fù)凍融抗體!
1.拿到抗體后請(qǐng)務(wù)必在1000-3000轉(zhuǎn)離心1-2分鐘后再打開管蓋進(jìn)行分裝和保存!(由于運(yùn)輸過程中反復(fù)顛簸,管內(nèi)微量抗體容易聚集在管蓋處,一旦直接打開容易流失抗體。請(qǐng)放心抗體出廠時(shí)保證已裝入足量。)
2.對(duì)于大部分抗體,比較合適的保存方式是分裝后保存在-20℃冰箱。
◇分裝可以最大程度降低反復(fù)凍融對(duì)抗體活性的損害,ErbB-2抗體同時(shí)也降低了多次從同一管中吸取抗體造成污染的可能性。
◇分裝的量以一次實(shí)驗(yàn)用完為好,但建議最少不要少于10μL每份。分裝體積越小,抗體濃度可能會(huì)受到蒸發(fā)以及管壁吸附的影響,同時(shí)分裝次數(shù)越多移液吸頭吸附的抗體也越多,可能會(huì)誤認(rèn)為抗體沒有足量裝入。(最好用無菌的進(jìn)口0.2ML離心管來分裝抗體,國產(chǎn)的小管一般沒有經(jīng)過很好的硅化處理,容易吸附抗體蛋白,影響抗體活性。)
◇分裝時(shí)請(qǐng)將無菌的微量移液器吸頭先插入管底部反復(fù)吹打幾次后再吸出抗體,ErbB-2抗體以便抗體有效成分與抗體保護(hù)劑充分混勻。
◇復(fù)融后的分裝抗體如果一次用不完,請(qǐng)將剩余母液保存在4℃冰箱,避免再凍起來!
◇絕對(duì)避免將抗體保存在自動(dòng)除霜冰箱的冷藏室中。盡量將抗體保存在手動(dòng)除霜冰箱里面,而不是在冰箱門上。
3.大部分抗體收到后4℃短暫保存1-2周對(duì)抗體活性是基本沒有影響的。如果抗體很快(1-2周內(nèi))就能使用完,推薦在4℃保存,避免反復(fù)凍融對(duì)抗體活性的損害。如果要長期保存則分裝后-20℃保存。
4.即用型抗體請(qǐng)務(wù)必保存在4℃冰箱,一定不能冷凍保存。
5.特殊抗體的保存:
◇酶聯(lián)抗體:一般保存在4℃,盡量避免冷凍起來。否則可能會(huì)導(dǎo)致酶活力的下降或者喪失。
◇偶聯(lián)抗體:所有偶聯(lián)抗體需要4℃避光保存。尤其是熒光標(biāo)記抗體,對(duì)光極為敏感,因此所有實(shí)驗(yàn)階段也是要避光操作的。
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關(guān)鍵詞:
ErbB-2抗體 ErbB-2抗體價(jià)格 ErbB-2抗體說明書