產(chǎn)品貨號(hào)XY0465-2
產(chǎn)品名稱
ERBB4抗體蛋白名Receptor tyrosine-protein kinase erbB-4
別稱HER4
ERBB4抗體免疫原多肽
來(lái)源兔
特異性抗人,大鼠,小鼠
克隆類型多克隆
應(yīng)用WB免疫印跡
成分200μg/ml親和純化抗體,0.01M PBS,30%甘油。
保存條件連續(xù)使用時(shí)4°C存儲(chǔ),保質(zhì)期六個(gè)月;長(zhǎng)期存儲(chǔ)時(shí)建議分裝為10ul以上小包裝-20°C存儲(chǔ),并避免反復(fù)凍融,保質(zhì)期一年。
背景資料This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
參考效價(jià):Western blotting: 1:100-400
Immunocytochemistry in fixed cells: 1:100-500
Immunohistochemistry in frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
ERBB4抗體的保存建議:
抗體保存得當(dāng)與否直接決定了抗體的活性和使用效果。如果抗體保存得當(dāng),大部分抗體活性都可以維持?jǐn)?shù)月甚至數(shù)年。請(qǐng)按照說(shuō)明書(shū)或者抗體標(biāo)簽上推薦的保存條件正確保存抗體!無(wú)論是保存還是運(yùn)輸,絕對(duì)避免反復(fù)凍融抗體!
1.拿到抗體后請(qǐng)務(wù)必在1000-3000轉(zhuǎn)離心1-2分鐘后再打開(kāi)管蓋進(jìn)行分裝和保存!(由于運(yùn)輸過(guò)程中反復(fù)顛簸,管內(nèi)微量抗體容易聚集在管蓋處,一旦直接打開(kāi)容易流失抗體。請(qǐng)放心抗體出廠時(shí)保證已裝入足量。)
2.對(duì)于大部分抗體,比較合適的保存方式是分裝后保存在-20℃冰箱。
◇分裝可以最大程度降低反復(fù)凍融對(duì)抗體活性的損害,ERBB4抗體同時(shí)也降低了多次從同一管中吸取抗體造成污染的可能性。
◇分裝的量以一次實(shí)驗(yàn)用完為好,但建議最少不要少于10μL每份。分裝體積越小,抗體濃度可能會(huì)受到蒸發(fā)以及管壁吸附的影響,同時(shí)分裝次數(shù)越多移液吸頭吸附的抗體也越多,可能會(huì)誤認(rèn)為抗體沒(méi)有足量裝入。(最好用無(wú)菌的進(jìn)口0.2ML離心管來(lái)分裝抗體,國(guó)產(chǎn)的小管一般沒(méi)有經(jīng)過(guò)很好的硅化處理,容易吸附抗體蛋白,影響抗體活性。)
◇分裝時(shí)請(qǐng)將無(wú)菌的微量移液器吸頭先插入管底部反復(fù)吹打幾次后再吸出抗體,ERBB4抗體以便抗體有效成分與抗體保護(hù)劑充分混勻。
◇復(fù)融后的分裝抗體如果一次用不完,請(qǐng)將剩余母液保存在4℃冰箱,避免再凍起來(lái)!
◇絕對(duì)避免將抗體保存在自動(dòng)除霜冰箱的冷藏室中。盡量將抗體保存在手動(dòng)除霜冰箱里面,而不是在冰箱門上。
3.大部分抗體收到后4℃短暫保存1-2周對(duì)抗體活性是基本沒(méi)有影響的。如果抗體很快(1-2周內(nèi))就能使用完,推薦在4℃保存,避免反復(fù)凍融對(duì)抗體活性的損害。如果要長(zhǎng)期保存則分裝后-20℃保存。
4.即用型抗體請(qǐng)務(wù)必保存在4℃冰箱,一定不能冷凍保存。
5.特殊抗體的保存:
◇酶聯(lián)抗體:一般保存在4℃,盡量避免冷凍起來(lái)。否則可能會(huì)導(dǎo)致酶活力的下降或者喪失。
◇偶聯(lián)抗體:所有偶聯(lián)抗體需要4℃避光保存。尤其是熒光標(biāo)記抗體,對(duì)光極為敏感,因此所有實(shí)驗(yàn)階段也是要避光操作的。
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關(guān)鍵詞:
ERBB4抗體 ERBB4抗體價(jià)格 ERBB4抗體說(shuō)明書(shū)